Abstract: 12.00 Effect Of Non-Expanded Adipose Stromal Vascular Fracture On Viability Of Transverse Rectus Abdominis Musculocutaneous Flap After Abdominoplasty: Experimental Study

MateRials and Methods: 35 male Sprauge Dawley rats were divided into 5 groups; each group consisted of 7 rats. Reverse abdominoplasty model was used in all groups except group 1(Only TRAM flap). Right inferior epigastric artery pedicled, 5x2.5 cm sized TRAM flap was performed 2 weeks after abdominoplasty in groups 2 and 4 and 4 weeks after in groups 3 and 5. ASVF cells were injected locally during abdominoplasty in groups 4 and 5. The viable flap area percentage was assessed by pixel count, newly formed perforators by surgical microscope, vessel count by microangiographic imaging, capillary density and fibrosis gradient by histopatological analysis and ASVF cells marked with DiI by fluorescent microscope. Additionally, plasma VEGF levels were measured.